I am trying to extract DNA from frozen newt skin swabs but have been getting really low concentrations (about 1-3 ng/µl). I am using the Qiagen Blood & Tissue Kit and just cut of the tip of the swab and let lysis go on overnight at 56°C and 400 rpm. I wonder if I should add a more rigorous shaking step? I know that I can't expect very high concentrations from this method, but I think it should be higher than what I have (e.g. Ringler, 2018: Testing skin swabbing for DNA sampling in dendrobatid frogs; she extracted 20-170 ng/µl in dendrobatid frogs). Does anybody have a useful hint what I could change to get higher concentrations? Thank you!
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