Elisabeth Mayr

4 Questions 4 Answers 0 Followers

Questions related from Elisabeth Mayr

How to extract DNA from skin swabs of Salamandra atra?

I am trying to extract DNA from skin swabs of Salamandra atra but the DNA concentrations I get are very, very low (like < 0,5 ng/µl). I am using the Qiagen QIAamp DNA Micro Kit in combination with...

23 August 2023 2,520 2 View

How do I extract DNA from frozen newt skin swabs?

I am trying to extract DNA from frozen newt skin swabs but have been getting really low concentrations (about 1-3 ng/µl). I am using the Qiagen Blood & Tissue Kit and just cut of the tip of...

18 May 2022 1,950 1 View

Does Sorbitol wash buffer need to be kept at 4°C?

I want to isolate DNA of old plant material tomorrow using a CTAB protocol. Unfortunately i forgot to put the sorbitol wash buffer (100mM Tris-HCl, 5mM EDTA, 0.35M Sorbitol, pH8) into the fridge...

18 July 2018 2,486 4 View

Does Sorbitol wash buffer need to be kept at 4°C?

I want to isolate DNA of old plant material tomorrow using a CTAB protocol. Unfortunately i forgot to put the sorbitol wash buffer (100mM Tris-HCl, 5mM EDTA, 0.35M Sorbitol, pH8) into the fridge...

07 July 2018 833 5 View