I want to isolate DNA of old plant material tomorrow using a CTAB protocol. Unfortunately i forgot to put the sorbitol wash buffer (100mM Tris-HCl, 5mM EDTA, 0.35M Sorbitol, pH8) into the fridge after autoclaving and hence it was kept at room temperature for about a week.
Is the only reason to keep it at 4°C to prevent bacterial growth and do you think I can still use it (it has not been opened after autoclaving)?
There is a yeast Genomic DNA Kit which contains sorbitol - they store it at room temperature
https://www.biovision.com/documentation/datasheets/K1387.pdf
Hi
Yes ,
you can use it. Just make sure, there is no any bacteeial or fungal growth or any turbidity in your buffer.
Thank you both for your answers! Since it appeared totally clear I used it for my extractions today.
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