Hi :)

I'm looking to expand MSCs from human BMAC, and then to subsequently characterise them. A lot of papers talk about 'washing' the BMAC with PBS prior to resuspending in media and plating. Would people recommend doing this, and what is the ratio of BMAC to PBS to use? I have a 4ml sample of BMAC and not sure if to wash it in smaller aliquots. Also, is it worth filtering the BMAC prior to washing?

Many thanks,

Sarah

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