I am attempting to isolate ovary from Helicoverpa armigera larvae. I can't find any pictures showing what it looks like during dissection. I know it is possible since there are several articles describing sequencing of gonad transcripts (ovary and testes). Any help? Ideally I would like to see some pictures of what the ovary looks like during dissection and where exactly it is located.
Thanks in advance!
EDIT: Thanks to Muhammad F Chaudhury for providing me the full version of his article titled " Spermatogenesis and Testicular Development of the European Corn Borer, Ostrinia nubilalis (Lepidoptera: Pyraustidae)". In it he describes that in this insect the testis of late-instar larvae are yellow-orange colored, reniform and aprox. 1mm in size.
This information allowed me to identify them in Helicoverpa larvae.
Hello
Kindly look at link here , hope help you
Article Molecular Characterization and Function Analysis of the Vite...
Good Luck
Sorry, I do not understand which might be the relationship between attmpts to isolate ovary from Helicoverpa armigera larvae and sequencing of gonad transcripts (ovary and testes).
I think you first check at which stage of development the larvae differentiate a gonad, next, you have to make seriate sections, carefully numbered, and see at the microscope the place where the gonadal tissue can be identified. Then, you will have an evaluation of its position.
Thanks to Muhammad F Chaudhury for providing me the full version of his article titled " Spermatogenesis and Testicular Development of the European Corn Borer, Ostrinia nubilalis (Lepidoptera: Pyraustidae)". In it he describes that in this insect the testis of late-instar larvae are yellow-orange colored, reniform and aprox. 1mm in size.
This information allowed me to identify them in Helicoverpa larvae.
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