The only reagent that I could use to see something is Blood reagent but the handling of this reagent is not as easy as anyother reagents. The fraction of such interest has been tried with spray reagents like Anisaldehyde-sulfuric acid, Ceric culfate, CAM reagent, etc to no avail. Any suggestion regarding a reagent that should stain the saponins in my fraction would be more helpful.
Hi, please try to find in the Camag TLC data base, I hope you can find suitable TLC's conditions and reagent for your compound. I got 155 hits if I insert "saponin" in that data base.
Best regards
http://www.camag.com/en/tlc_hptlc/customer_magazine_cbs/ccbs_database.cfm
Hi,
Liebermann-Burchard reagent or ethanol-sulfuric acid (90:10) can be used to detect saponins. Both function like anisaldehyde-sulfuric acid and blood reagent for saponins identification.
Hi, I usually use a mixture of water acetic acid and sulfuric acid (10:73:17) that works for saponins and other metabolites as well
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