How do I detect Chondroitin sulfate proteoglycans by immunohistochemical method in spinal cord?

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How do I design MTT for enaluation of growth factor effcts for fast growing cells?

Hello everyone I am trying to design an MTT experiment for an immortal adherent cell line with doubling time of 20 hours. The goal is to evaluate effects of certain growth factors and their...

13 February 2024 3,162 4 View

What is the preferred medium for expansion of Mesenchymal Stem Cells?

I have read some papers on MSC isolation from human adipose tissue. DMEM and DMEM/F12 medium have been used for expansion. I need to know which type of DMEM (low/high glucose) is preferred for MSC...

05 February 2024 9,290 6 View

The problem of writing a Sas command to analyze a factorial design in a completely randomized format in two years?

The problem of writing a Sas command to analyze a factorial design in a completely randomized format in two years Factorial design in a completely random format with 3 factors that was implemented...

25 January 2024 1,565 2 View

Who needs a remote professional academic writer in molecular genetics?

Sincere greetings My academic English writing is excellent. I desired to join several active groups within my field. My concentration is in molecular genetics. But I was unable to locate any. I...

20 October 2023 7,629 2 View

How to analyze qualitative data on Instagram?

Hi everybody! I intend to conduct qualitative research (content analysis using MAXQDA) on identifying factors affecting the success of sales pages on Instagram. The goal is to identify the common...

23 August 2023 255 4 View

How to prepare a distribution map of Fritillaria genus in the world?

Fritillaria is one of the largest genera of the Liliaceae family. There are about 170 taxa of this genus all over the world. These are mostly native to the temperate regions of the Northern...

03 July 2023 5,678 0 View

Can you please help me identify the unknown peaks in the FTIR spectrum?

Two peaks are observed in the region of 1570 and 1540cm-1. These peaks can be related to what functional group? And seeing a sharp peak in the 3600 area is related to what?

11 May 2023 7,947 6 View

What is the data about electro etching of in738LC super alloy?

the voltage and the chemical composition of electrolyte and also the time, to reveal the secondary dendrite arm spacing.

26 April 2023 4,438 0 View

Measuring starch content of an aqueous sample?

I have some samples from microbial fermentation of pea flour. They are aqueous samples and contain solubilized starch (the bacteria use the starch as their carbon source). I want to measure the...

17 April 2023 8,238 3 View

Anyone expert in Aspergillus organelles?

Would you please message me to assist me in detection of some modified organelles in Aspergillus ? If you are able, please drop me an Email: [email protected] Thanks in advance

01 March 2023 6,558 3 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

Who will be moral responsible for the death of thousands of people in the event of an earthquake?

Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...

08 August 2024 6,134 12 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View