Hi,

I have made multiple attempt to cultivate DENV2 and DENV3 virus from frozen stock using vero cells. ATCC recommended using LLC-MK2 cells, but since I don't have the cell line, I figured I could use vero cells since it is known to be infected by the virus. I had to get a new vial of the virus from ATCC after my first attempt didn't work.

Does anyone know how to go about this and how long DENV incubate for upon the first cultivation from frozen stock