Why is WST-1 assay giving inconsistent result?

i want to determine the cytotoxicity activities of some drugs with WST-1 assay using 293T cell lines in 96 well plate. I usually seed 10,000 cell per well and incubate for ~48 hours. I expected...

01 February 2021 8,554 3 View

Can you do a research collaboration with me on building feeling into telepresence devices?

We need to move from virtual reality to real world again. The research aim at making individual working on a robot at a distance to feel what the robot feel order than by vision and tactile...

08 June 2020 2,575 6 View

Nanoparticles in lubricants ?

How is it that we propose nano particles in lubricants, for instance, engine oil. To what extent does the particle size in relation to viscosity affect the metal surfaces. Is it a trade off due...

03 February 2020 7,385 4 View

Which one is better to use for water stress experiment GWAS or RNASeq?

Hi everyone, This is my first time in this area of research. I am working on 95 varieties of bambara groundnut. I have done the agromorphological characterization of these varieties as well as the...

09 September 2019 3,782 4 View

Problematic blunt end restriction enzyme digestion?

i have tried so many times to perform a partial digest with MsAp1I restriction enzyme (creates blunt end) on F88-4 phage vector(9234bp), it has 2 sites which overlaps a CpG site, which i read...

05 March 2019 6,451 2 View

How can i set up a meaningful drought experiment?

Hello, I want to set up a drought-response experiment. Should i consider using the green house or the field. Secondly, will 100 accessions of this crop be too much for the experiment? Lastly, will...

06 August 2018 1,424 6 View

Where can I get a functioning SEM Machine?

My current work is on microstructure of bio ash stabilized soil. I need to do a scanning electron microscopy to identify the reaction products.

07 May 2018 757 6 View

Hello everyone, please what is the best protocol for extracting secondary metabolites from rhizosphere bacteria?

I am working on rhizosphere bacteria and need to identify the secondary metabolites they produce which aid in their biocontrol ability. Thanks

30 August 2016 8,885 3 View

What is the limit of cyanide concentration in the soil that is not a threat to the environment?

25 August 2016 8,852 3 View

Validation of mathematical/analytical Models

The acceptable practice is to validate a model used under any study. What validation methods are available and would we confirm one superior over others?

01 January 1970 5,708 19 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View