I am preparing a research plan that involves synergy of phytochemicals, my main area of interest in Lipid metabolism but I also want to demonstrate the effects on the obesity induced inflammation without having to use a different set of test animals or cell cultures. How can I perform the two together? Is testing the levels of circulating Cytokines good enough for such a case?
You can also perform flow cytometry in blood as well as organs of interest, such as the liver or adipose tissue and measure levels of various immune cells. You can perform immunofluorescent staining on organ sections and look for cellular composition - check for inflammatory cells such as macrophages. Moreover, you can perform ELISA on organ homogenates (liver, adipose tissue homogenates) in addition to plasma for inflammatory cytokines. In those homogenates you can also measure lipid levels, cholesterol&triglyceride, you can look for lipoprotein fractions and you can also look at lipid content of cells in organ sections.
Hi, Kevin Odongo.
Answering your question, it may be a good way. But, in order to evaluate it a little bit better, you need to give some better insight into your idea.
Firstly, I would say talking about your project to other scientists openly may not be a good idea. But if you still want to talk about, my first question would be:
what inflammation disease model you're wondering to work with? Because based on your answer, systemic evaluation may not be the best option. Let's say: If you decide to work in osteoarthritis, local inflammation may give you best results then systemically. Got it?
Hope it helps.
You can also perform flow cytometry in blood as well as organs of interest, such as the liver or adipose tissue and measure levels of various immune cells. You can perform immunofluorescent staining on organ sections and look for cellular composition - check for inflammatory cells such as macrophages. Moreover, you can perform ELISA on organ homogenates (liver, adipose tissue homogenates) in addition to plasma for inflammatory cytokines. In those homogenates you can also measure lipid levels, cholesterol&triglyceride, you can look for lipoprotein fractions and you can also look at lipid content of cells in organ sections.
- Badges
- Science topic