There is a protocol with DNAse treatment, but it is labour intensive and doesn't allow to clean large number of the elecrodes. I thought about how sonification bath can be helpful in this case, but I couldn't find appropriate protocol.
I am trying to find RCN in real-time PCR on SYBR Green, but Cq among 3 repetition of the same DNA is going up and down around 1.2 cycle, so the error is too large. I'm working on genomic DNA...
05 October 2023 957 5 View
Willamson R.B. (1974). Further captures of Pacific salmon in Scottish waters. Scottish Fisheries Bulletin 41, 28-30. Pyefinch K.A. (1962). Capture of pink salmon on the Scottish coast. Ann Biol...
09 March 2023 5,519 2 View
Hello everyone, I am looking for an open dataset to verify the results obtained by analyzing the total RNA-seq of patients in the TARGET-AML project (GEO search was not successful). The dataset...
03 July 2022 2,545 8 View
I am trying to figure out how to create a map representing probability of some phenomena occurrence. I have a binary raster (1- phenomena exists; 0 - doesn't exist) and several raster that explain...
07 March 2021 5,642 5 View
What media or salt solution (HBSS, EBSS, Tyrode) can be used for real-time intracelular h2o2 imaging? I am using a genetically-encoded biosensor based on roGFP and have to find the media that...
28 October 2020 7,503 2 View
I'm quite new to VAMPIRE. Any suggestions for finding resources and example codes?
24 December 2019 8,187 2 View
I have a question about spdep package, ‘impacts’ function (in R). I’ve estimated spatial model (panel data). With this function I can calculate average direct and average indirect effects...
15 September 2019 5,483 2 View
Can you please help me to explain each event on this DSC curve. I suppose these peaks are due to the following reasons: 1. The first exothermic peak on the cooling curve is when crystallization...
04 May 2019 1,298 3 View
Greetings to you, dear colleagues! Need help in finding articles on sterlet parasites and sturgeon. Who can help, send me please! Thank you in advance.
18 April 2018 3,019 4 View
I've decided to change the delivery methods for CRISPR/Cas9 system from plasmid to ribonucleoprotein (RNP) complexes. According to some souces, this method gives higher % of modifications in...
05 July 2017 193 11 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it
06 August 2024 947 1 View
Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...
04 August 2024 8,997 1 View
Hi everyone I need a file with a dirty and clean potato image
04 August 2024 7,199 4 View
The module of the CAD detector is corona Veo. It has been idle for a long time. And it was not well maintained before idle. I have replaced the gas filters and flushed the system with a 50/50...
01 August 2024 7,805 0 View
We are working on a robot that cleans solar panels using fresh water supply and a rotating brush. We are trying to conserve as much water at possible by recycling the dirty water that is collected...
28 July 2024 5,778 2 View
I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...
28 July 2024 8,027 3 View
Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...
10 July 2024 5,985 0 View
Handbook [of] Heat Exchanger Fouling: Mitigation and Cleaning Technologies Hans Müller-Steinhagen IChemE, 2000 - Technology & Engineering - 382 pages
29 June 2024 8,259 0 View
If I dope silver during the synthesis of copper indium sulphide nanoparticles by hydrothermal method what will be the size of nanoparticles?
13 June 2024 6,844 3 View