Hello everyone!!
Currently, I am working on the fabrication of PLA nanoparticles with PEG adsorbed on their surface. I am based on the article "The internalization of fluorescence-labeled PLA nanoparticles by macrophages" where they used BASDE as surfactant and CHCl3 as solvent, but my problem becomes after the centrifugate process, because I got a sticky pellet impossible of resuspend in water...
I tried to change my solvent for DCM but the result was the same...
I was thinking in use PVA instead BASDE but I'm not sure about the real problem that is causing this...
I appreciate your help!