Hi community,
Maxquant features the option to add chemical labels, isotopic labels and etc. but is there a way to define selfmade chemical labels? Is this process complicated to implement?
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
01 March 2021 9,447 3 View
I have this set of data: Treatment 1: 30.5; 34.5; 24.4 Treatment 2: 24.8; 20.8; 16.8 Treatment 3: 19.1; 21.4; 21.0 Treatment 4: 22.3; 26.1; 27.1...
25 November 2020 920 4 View
I'm studying for a master's degree. I'm doing research in 3D printing by using metallic glass powder... I would like to buy some to do experiments. It would be very helpful if anyone can suggest...
29 October 2020 184 4 View
I adopt primer designs from a study published in Nature ( Domestication selected for deceleration of the circadian clo... ), so the primers should be qualified. Yet in many samples, I got...
31 August 2020 9,796 8 View
Hello all, At the moment, I am working on a project about the failure/fault detection on Photovoltaic Module based on Deep Learning using electroluminescence (EL) images. In this project, my...
23 August 2020 2,832 4 View
Dear all, I want to conduct a network meta-analysis to find out the efficacy of different surgical interventions. I will use R as the main software. However, this is the first time I do a network...
21 June 2020 8,424 4 View
Hello scientists, We are proposing to develop a potential fault diagnostic model of Power Transformers according to the approach based on Hedge Algebra. Following this approach, we need to have...
03 April 2020 1,743 3 View
I have data like this one: Study__ Periods_Control_Experiment Study1 1990-2000 100 50 Study2 1992-2005 78 90 Study3 1986-2009 98...
29 March 2020 7,330 3 View
Did anyone transform un-digested pPICZalpha-A (circular DNA) which was inserted a desired gene into Pichia pastoris using electroporation? According to User Manual's Invitrogen (EasySelect Pichia...
01 March 2020 6,604 3 View
I've been preparing an oral dispersible film of a insoluble drug (Candesartan cilexetil). I used HPMC E5 as film forming, PEG400 as plasticizer. I tried using solid dispersion method taking...
19 February 2020 1,515 1 View
An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...
02 March 2021 9,661 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...
27 February 2021 428 3 View
Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...
25 February 2021 1,675 3 View
I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...
23 February 2021 6,756 1 View
a free multi label clinical text dataset , containing text as entry and several labels as classes. Best regards
22 February 2021 5,988 2 View
New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...
21 February 2021 3,278 3 View
I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...
18 February 2021 4,392 4 View
Hello, it is about creating a dataset for semantic segmentation with three classes. The problem is that one class dominates with >90% and one class is
18 February 2021 4,254 5 View
Good evening everyone. If a questionnaire has 4 factors measuring different constructs as for example CAMI questionnaire measuring attitudes toward the mentally ill that has four factors:...
18 February 2021 3,223 3 View