Hi community,
Maxquant features the option to add chemical labels, isotopic labels and etc. but is there a way to define selfmade chemical labels? Is this process complicated to implement?
I studying PhD course in Urban design for Health. I need to know in some fileds: residental block, green open space and Transport TOD. I need connect with expert in that fileds.
14 July 2024 1,892 3 View
Does anyone have experience with plant meristem culture? What kind of plant growth regulator combination is best? From what I read, there seem to be 2 methods. One using cytokinin (BAP, kinetin)...
08 June 2024 7,877 2 View
For phylogenetic analysis using SSRs, normally we don't use just one SSR, but a set of multiple SSRs to ensure accuracy. So how can we input these data into NTSYS? I know input for one SSR is as...
13 May 2024 1,867 1 View
Dear everyone, I am currently making a recombinant protein (protease) for checking its activity by zymography. However, before column purification (his-tag), the activity was shown only one band...
04 May 2024 1,197 3 View
Hello, I have a question related to the amount of total protein can be extracted per one ovary of mouse (ICR strain). Do you know how much protein we can get (maximum) per one ovary of mouse...
05 April 2024 4,720 0 View
Hi all. I know that crystal field splitting occurs in octahedral complexes in magnetic materials. However, I'm unsure whether crystal field splitting depends on temperature. Can crystal field...
28 March 2024 452 1 View
My experimental product is a base shower cream by mixing the fatty acid with KOH at 75 degrees Celsius and other ingredients (hydroxypropyl methylcellulose (HPMC), emulsion chemical, salt,...),...
12 March 2024 8,769 0 View
In all academic sources, sucrose is identified as α−glucose (1-->2) β−fructose. However, I cannot find any explanation anywhere as to why the fructose monomer has to be in the β configuration....
12 March 2024 9,230 4 View
After performing PCR, I ran electrophoresis, but on agarose the results showed some rather blurred samples. I wanted to know the cause and how to fix this situation. Please note that the chemicals...
05 March 2024 8,253 5 View
Dear all, I created a profile a long time ago with 2 publications included in that profile. Now I have a new profile and would like to merge two profiles into one, or at least I can include those...
17 February 2024 5,904 1 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Since 2016 Brexit, the world needed to change the thinking behind traditional democracy as the democratic landscape changed, yet traditional democratic thinkers and actors have been acting as if...
28 July 2024 6,515 1 View
Do You Believe VP Harris Would Show Empathy to Tech Companies in Her Administration as POTUS? I ask in light of the fact that, the ACP was not re-funded this round. So many and businesses need...
24 July 2024 4,732 3 View
Please suggest basic literature on lattice-based cryptography. A kind response from the cryptographic community will be highly appreciated.
24 July 2024 6,291 4 View
I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?
23 July 2024 8,059 1 View
The above are manually labeled extrinsic matrices based on the first image It can be seen that the projection error at the edge is large, while the error at the center is small. What could be the...
23 July 2024 7,479 3 View
how do i set one up? What do I include?
22 July 2024 6,097 4 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
I have attempted various methods, including homozygous gene disruption and CRISPR-Cas9, but have not achieved the desired results. It appears that the gene of interest is situated in a...
15 July 2024 3,977 1 View
Before entering a project, social license and consent from the community of the said area is very crucical as it determined whether the projects is accepted and viable to the community and...
14 July 2024 5,319 0 View