I need to extract (or distill) queen pheromones from queen bee mandibular glands. Are there any methods for doing this? I am conducting an experiment pheromone attract and repel.
Hello, Fill a vial or other small glass or plastic container with cotton wool. Combine 10 drops of lemongrass essential oil with 5 drops of geranium and one drop of lemon or lime in a bowl and stir. Drip the mixture into the vial. You can use a bit of cotton wool to soak up the last traces.
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Hello, Fill a vial or other small glass or plastic container with cotton wool. Combine 10 drops of lemongrass essential oil with 5 drops of geranium and one drop of lemon or lime in a bowl and stir. Drip the mixture into the vial. You can use a bit of cotton wool to soak up the last traces.
How to extract the same topic well discussed here. . .
https://www.google.com/amp/s/www.pinterest.com/amp/pin/181129216237441314/
Good luck!
Hello, Martin. Take a look at the early work of Winston et al.Article The influence of queen mandibular pheromones on worker attra...
I hope it helps.
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There is a great number of pheromones in the queen bee's body, but I assume you mean queen mandibular pheromones (QMP). These are found in the mandibular glands present in the head. To extract them you need either to dissect out the mandibular glands or to extract the whole head. The latter is easier -- you just separate the head from the body and put it into a vial with methanol, ethanol or any other polar solvent. However, this will give you not just the QMP, but also the contents of other head glands and possibly some lipid from the fat cells. Dissecting out the glands will give you just one type of pheromone. If you want ALL pheromone types at once (QMP, Dufour's, tergal, etc.) you can do a whole body extract -- put the whole queen in a vial of solvent and leave it for around 10 minutes. This way has some disadvantages. First of all if you leave the queen in solvent for too long you will extract fat body lipids as well, but a short exposure will not give you adequate extraction of glands. Also, to extract different types of glands you need solvents with different polarity.
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