How do extract/distill queen phermones from queen bees?

I need to extract (or distill) queen pheromones from queen bee mandibular glands. Are there any methods for doing this? I am conducting an experiment pheromone attract and repel.

07 August 2019 3,038 5 View

How do I determine magnification on Nikon SMZ460?

We just purchased three Nikon SMZ460 stereo scopes. They have 20x eyepieces and no auxiliary objective. We are looking at small insects. There is too much magnification. We can only see one insect...

10 November 2018 1,068 3 View

Is there a histology stain for staining the polar tube in Nosema apis and/or N. ceranae?

I am looking for a protein stain for Nosema apis and/or N. ceranae polar tubes for brightfield or fluorescence microscopy.

09 October 2017 696 1 View

Is there a turn key software package for studying pathogen morphology?

Does any know of a turnkey software application for distinguishing different morphologies of pathogens in the same genus? i.e., I have two microsporidial pathogens that have slightly different...

09 October 2016 8,913 2 View

Is there a relationship between music and nature?

Has anyone examined the relationship between music and honey bee stress and/or brood production?

07 August 2016 2,643 0 View

Anecic worms

Is anyone in the United States studying the relationship between anecic worms and soil structure?

11 December 2014 4,182 0 View

Is anyone doing any histo staining of the honey bee ventriculus in healthy honey bees and bees infected with Nosema ceranae?

Looking for stains that bind calcium.

03 April 2013 8,574 4 View

No title

02 March 2021 3,060 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View