Are there papers on?

I'm looking for a papers concerning either Christianity or neopagan religions and social media. What role does social media play in the spread of these religious movements? Any suggestions on the...

15 February 2021 655 4 View

Which commercial DNAse do you recommend me?

Which is the best DNAse (or DNAse kit) you have worked with? I am looking for a good DNAse that can remove DNA from an RNA sample in one pass. Material would be used for future RT-qPCR analysis....

18 November 2020 8,769 3 View

How to extract big peripheral membrane proteins?

I have been trying for a long time to see by Western Blot a 450 kDa-peripheral membrane protein from the Golgi apparatus with no success. I know that the electrophoresis and the membrane transfer...

28 September 2020 9,664 3 View

Maiden vs married name?

When I claimed authorship of papers written under my maiden name, they have now appeared under my married name, and I am worried this will cause confusion. How do I fix this issue?

12 August 2020 6,936 5 View

Rat DRG survival issue - how can I debug the protocol?

Hello everyone, I have been trying to establish primary rat DRG neuron culture for a couple of months already. I am using the protocol I found somewhere during the Google search, apparently...

14 June 2020 343 1 View

How to clean analytical size exclusion column?

Hello, We are using Superdex75 increase 3.2/30 GL size exclusion column in our lab and recently column pressure is to high to work on recommended flow rate 0.1 mL/min. I tried to clean the column...

19 May 2020 8,549 4 View

Unexpected problems running Photoquad software: How to fix the issue when it doesn't run anymore?

Hi, I've been using Photoquad software to analyze photoquadrats but suddenly the program doesn't run anymore and I am not able to solve the problem. I uninstalled both the MATLAB Compiler...

21 April 2020 4,707 1 View

Did any of you use polyethylene glycol to break down antibodies in Western Blot?

Hi, I've got a problem with my Western Blot. I have a low weight protein to detect but I suspect that my primary and secondary antibodies are clumping into a large ball at a higher molar mass than...

09 March 2020 2,856 3 View

Can anyone tell me the results of European Xenome research?

I know that the European Union has funded XENOME, an integrated project within the Sixth European Framework Programme (Life Sciences, Genomics and Biotechnology for Health), lasting 5 years...

18 November 2019 6,532 2 View

Hi all, is it possible to use XBridge BEH Amide Column to separate acetoin and 2,3-butanediol via HPLC?

Most of the methods that I have found recommend Aminex HPX-87H but I would rather avoind buying new column. Thanks, Marta

13 November 2019 6,221 2 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

How the standard hydrogen electrode value is convertible into reversible hydrogen electrode?

As much I remember +0.0592*pH, as I am working on CatMAP, so my voltage values are in SHE, not RHE.

18 February 2021 1,685 3 View

Can explain this diagram to me, is this the average consumption curve for one day or the total consumption of days in a month summed up in specific?

The hourly load curves of the selected household in each season are shown in Fig. 28. Figure 28 shows the demand for electricity in four months on an hourly basis. Can you explain this diagram to...

08 February 2021 657 16 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Which one is better for storing energy: Fuels synthesized from CO2 or Hydrogen?

Based on educated guesses, which one of the following promises a better future for storing energy for grid scale, automobiles etc as opposed to batteries: 1) Fuels synthesized form CO2 2)...

02 February 2021 8,741 6 View

Why is WST-1 assay giving inconsistent result?

i want to determine the cytotoxicity activities of some drugs with WST-1 assay using 293T cell lines in 96 well plate. I usually seed 10,000 cell per well and incubate for ~48 hours. I expected...

01 February 2021 8,554 3 View

How to perform hydrogen underpotential in hydrophobic material?

I have a working electrode composed of deposited graphene material (confirmed from Raman).The graphene is porous and has deposited Pd nanoparticles. I am trying to perform hydrogen underpotential...

01 February 2021 3,199 1 View