I am trying to detect ATM (a very high protein) by Western Blot; I have transferred overnight at 4ºC and at 40mA but I have not been successful. Any suggestion??
Hi I have the same trouble as you, were you able to solve it in the end?
I found this tips for HMW proteins:
https://www.alomone.com/info/western-blot-protocol-for-high-molecular-weight-hmw-proteins
Here they suggest a transfer with 100mA for 20h
How long did you run your gel?
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