CRISPR/Cas9 system for genome editing.
Thank you sir
I have AFM scans of a uniformly textured surface of 16x16um size. I would like to obtain the slope distribution of the surface using Nanoscope, as I plan on trying to relate this parameter to the...
10 April 2024 5,024 2 View
Over the last 10 years, I have been using the Mendeley citation manager. Due to their new policy, it is not working as smoothly with MS Word. Therefore, I am looking for a good alternative.
10 January 2024 3,460 5 View
In CRISPR/Cas, we design guide to make double strand break in a specific site of the genomic DNA. I am curious to know, whether the gRNA-Cas9 complex has any possibility to bind to the messenger...
25 December 2023 630 1 View
If I want to knock out an essential gene from E.coli genome and replace it with a homologous gene from another genus. What should be the strategy I Should follow
25 October 2023 3,773 2 View
I am trying to simulate FSW in CEL using abaqus. I am trying to replicate exactly the same simulation of this paper :Coupled Eulerian Lagrangian finite element modeling of frict... . But the...
04 August 2023 3,782 0 View
in the model I am simulating, the mixture of ethylene glycol and water is flowing in the hollow fiber membrane (a cylindrical hollow tube made up of porous media). during the flow, the water in...
19 July 2023 7,116 1 View
I have been trying to simulate the Friction Stir Welding process in Ansys LS-Dyna using the SPH method. I am struggling to couple it thermally to find the temperature, add convection, etc. Please...
12 July 2023 8,181 0 View
I am trying to simulate Friction Stir welding using the CEL method. I have resolved all other errors except this particular error. Someone kindly help me with this. I have attached the CAE file.
11 July 2023 8,959 2 View
which density should be used if you have material as PVC FOAM of 2 densitys 60kg\m^3 and another one of 80kg\m^3 which are material used to make seat for 2 vheeler (vechcial like activa )
10 July 2023 8,728 2 View
I am analyzing the gene sequence of my target gene in Brassica juncea. In that there is some ambiguities in the gene sequence which is appearing after few clear peaks in the sequence. I am...
27 June 2023 9,927 1 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?
31 July 2024 5,087 4 View
What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?
31 July 2024 7,921 5 View
How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?
31 July 2024 8,939 5 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.
26 July 2024 1,469 1 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
