We need to study the health of living trees before fructification on it. Does anyone know the procedure or DNA kit to extract fungal DNA from fresh woody samples?. Thank you beforehand
You need to take fungal sample and either mince/chop it with sterile blade or homogenize using liquid nitrogen. For further procedure you can use Qiagen blood and Tissue DNA kit as per the protocol provided in kit. This gives best yield.
Thank you Ali and Yashwant for your comments.
Samples will be taken by perforation of the living trunk in living palms to evaluate the presence of fungi inside the wood and detect future decaying of the palm which has been several times damaged with endotherapy holes.
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2
https://www.balticforestry.mi.lt/bf/PDF_Articles/2010-16[1]/BF10161%2035_42%20Verbylaite%20et%20al.pdf
4
https://www.woodworkslibrary.com/repository/structure_and_function_of-wood.pdf
5
https://www.ippc.int/static/media/files/publications/en/2013/10/02/2013_ifqrg-11-10_fraxback_malmo_sweden_2013.pdf
6
https://forskning.ku.dk/find-en-forsker/?pure=files/21206899/wp_43_wse_2009...pdf
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