How could I conform promoter SNP is a novel variation or known variation?

More Deepthi Pasumarthi's questions See All

Can anyone suggest Research statistical Techniques Book for Psychological research?

Dear Professionals and eminent Professors Recently, I participated in an international conference (webinar) for presenting my paper on positive psychology research. I could see other...

27 April 2024 4,901 3 View

Is the satellite hyperspectral data mosaiced or stitched?

Is the multispectral and hyperspectral satellite data that we downloaded from the USGS website, mosaiced or stitched? What percentage of overlap is used? Where can we find out this information?

14 March 2024 6,224 4 View

How to compare the USGS spectral library with MNF data in hyperspectral image techniques using ENVI software?

In pre-processing, when transforming hyperspectral data to a Minimum Noise Function(MNF) data, the dimensionality will reduce and at the same time the wavelength information will loose. B'z MNF...

26 February 2024 8,059 6 View

Finding Carbon Sequestration from remote sensing data?

How can I use an allometric equation to calculate the carbon sequestration of a forest using Remote sensing data? 1. If each species requires its own unique allometric equation. 2.How can an...

29 October 2023 6,473 3 View

How do you determine whether there is a protein contamination present in the given DNA sample? If so, what is the method to remove the contaminants?

Dna extraction from plant cells

14 June 2023 7,693 0 View

Can anyone share their work on "adolescent suicide interventions?

Esteemed RG Professionals I am in need of global level interventions or therapies available for treating adolescent suicidal behaviour. I am preparing a chapter on this for a handbook on child...

29 March 2023 2,410 0 View

How can we analyze lcms data with mz and rt time. Is it necessary that RT should match with the other research publications that has same mz value?

Kindly let me know to analyze LCMS data and identify secondary metabolites.

09 August 2022 8,693 2 View

Can anyone suggest free Plagiarism softwares?

Dear professionals I have checked many reviews on plagiarism softwares in the net but are not clear of which to choose. I need an authentic free plagiarism software with report. I value the...

23 December 2021 7,300 16 View

Difference between powder metallurgy and compression moulding?

For polymer powders in some articles it was mentioned as powder metallurgy and in some articles, it was compression molding. For polymers, is both are same

30 September 2021 5,911 4 View

My PTFE composite heat of fusion is 72 j/g and heat of crystallization is 36j/g. Why should so much variation?

After melting peak, during cooling process, crystallization peak has obtained. Why so much difference in values . what are the losses

15 August 2021 2,094 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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01 August 2024 2,079 0 View

Why Powder XRD is performed after cruhsing a crystal if Single Crystal XRD analysis of the same crystal has been performed already ?

What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?

30 July 2024 6,261 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to know the b axis of a single crystal?

How to apply magnetic field parallel to b axis of any crystal

29 July 2024 4,083 2 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

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Can anyone help me explain my problem?

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26 July 2024 2,136 0 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Frederic Lepretre

Hi Deepthi

so many ways to answer your question, the best for you for this topic and future plans would be to go to the UCSC website (http://genome.ucsc.edu), choose the genome browser tool with your species and position of the variant and see in the extra database if it's already exists.

if you don't have the genomic position, blast a part from your sequence containing the variant and you'll find the position.

all the best

fred

Deepthi Pasumarthi

Frederic Lepretre Tq for giving an answer .I will check in UCSC GENOME BROWSER

Laith Ali

By using genome browser