Deepthi Pasumarthi

5 Questions 5 Answers 0 Followers

Questions related from Deepthi Pasumarthi

How could I conform promoter SNP is a novel variation or known variation?

I am working on a promoter sequence of a gene to find SNPs for my project, for that, I gave sanger sequencing for some samples and I found one variation in my samples. How can I check this...

29 March 2021 998 3 View

How to calculate Unknown protein concentration based on standard curve by using BSA method?

I made curve for standard protein of BSA 125-1000 ug/ml. I draw linear line in Excel I got m value and c value. which can be generated by plotting the std conc in Xaxis and absorbance in Y in...

19 July 2019 9,523 1 View

What is the best housekeeping genes in humantissue and blood samples for qpcr analysis?

we are working on cervical cancer ,we would see the expression levels of target genes in both control and patient samples. we are planning to use internal control as Coiled-coil-helix-coiled...

22 December 2016 4,382 7 View

What is the best DNA extraction method for NGS Analysis?

I would like to know what the best DNA extraction method for NGS analysis generally we use phenol chloroform method for Cancerous tissue DNA isolation but i heard phenol inter pt the NGS...

12 February 2016 3,578 6 View

How to adapt the cells from RPMI TO DMEM medium?

I work with Hella in RPMI 1640. I need to change the medium of this cells from RPMI to DMEM. How to change these cell lines to adapt the cells to the new medium?

06 February 2014 3,619 12 View