Dna extraction from plant cells
Dear Professionals and eminent Professors Recently, I participated in an international conference (webinar) for presenting my paper on positive psychology research. I could see other...
27 April 2024 4,901 3 View
Is the multispectral and hyperspectral satellite data that we downloaded from the USGS website, mosaiced or stitched? What percentage of overlap is used? Where can we find out this information?
14 March 2024 6,224 4 View
In pre-processing, when transforming hyperspectral data to a Minimum Noise Function(MNF) data, the dimensionality will reduce and at the same time the wavelength information will loose. B'z MNF...
26 February 2024 8,059 6 View
There is a growing trend in research articles' authorship varying from five in number to anywhere around 15 which I have noticed. How are these articles cited in-text (as the general rule was to...
16 November 2023 9,677 4 View
How can I use an allometric equation to calculate the carbon sequestration of a forest using Remote sensing data? 1. If each species requires its own unique allometric equation. 2.How can an...
29 October 2023 6,473 3 View
Esteemed RG Professionals I am in need of global level interventions or therapies available for treating adolescent suicidal behaviour. I am preparing a chapter on this for a handbook on child...
29 March 2023 2,410 0 View
Kindly let me know to analyze LCMS data and identify secondary metabolites.
09 August 2022 8,693 2 View
I wanted to conduct a study to assess the effect holistic nursing among patents undergong maintainance hemodialysis
05 June 2022 6,461 3 View
Dear professionals I have checked many reviews on plagiarism softwares in the net but are not clear of which to choose. I need an authentic free plagiarism software with report. I value the...
23 December 2021 7,300 16 View
For polymer powders in some articles it was mentioned as powder metallurgy and in some articles, it was compression molding. For polymers, is both are same
30 September 2021 5,911 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
