How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

How big the Balb/c mice spleen after immunization?

I am working with Balb/c mice, and in my experiment, I have to inject mice with antigens, then get their spleen and collect the splenocytes for fusion. Recently, I get into trouble with the mouse...

04 January 2021 3,591 3 View

Capture of oleophilic solid polymers present in water solution ?

I am looking for articles, coferences, publications or thesis concerning the capture of solid oleophilic polymers in solution in water. Thanks Thierry Le Blan

01 January 2021 5,798 3 View

Hybridoma stops producing antibodies after thawing from liquid nitrogen?

Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...

01 December 2020 1,178 3 View

Suggestion for normal ovary cell lines?

Dear all, I want to choose a normal ovary cell line and check for its proliferation property when introducing carcinogenic factors. Does anyone have any suggestions for the cell line? Many thanks...

08 November 2020 9,100 3 View

What is Snapback Primer in realtime PCR?

Dear all, I find a paper using Snapback primer. I do not understand the function of snapback tail? What is snapback primer? When do we use snapback primer? and why we use that? Thank you.

01 November 2020 8,869 2 View

Rotation matrix - 3D dimension to 2D dimension?

22 October 2020 9,354 4 View

Am I introducing any potential loss of RNA integrity by combining an RNALater solution with a non-ionic detergent?

I am planning to characterise the transcriptome of pre-anthesis kiwifruit anthers. The samples are being harvested on my behalf and sent down by post, and I cannot ask the harvesters to dissect...

10 October 2020 1,830 3 View

Multilevel model for change- using time-varying variables at different waves to outcome?

I am using the multilevel model for change. I am using UKHLS youth survey where an outcome for wellbeing occurs at waves 2,4,6 and 8. I have predictors such as computer screen usage and many...

28 September 2020 3,984 1 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

Any reference paper with detailing on how to hybradize Q-Learning algorithm with Genetic Algorithm, especially for a variable optimization problem?

I am planning to hybradize Q-Learning algorthm with Genetic algorithm at the population initialization stage, crossover stage and mutation stage. The problem under consideration is a...

07 February 2021 3,123 8 View

How can I transform 50 kb plasmid into ecoli?

I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...

22 January 2021 8,612 3 View

How can I get the chromosomal location of a list of genes?

I'm looking for a bioinformatics website to get the chromosomal location of a radish gene id list, but in one shot instead of doing it gene by gene. Is there a way to do this? Thanks

17 January 2021 8,810 3 View

Are you equipped to count small plasmid chromosomes?

I wish to perform an experiment which will provide support for a novel DNA structure. Most of the experiment is conceptually simple, easy to perform, and inexpensive. But the key to doing it is...

29 November 2020 5,258 9 View

What is this citation style called?

Hello everyone, I am preparing a manuscript for a journal which indicates in the authors guidelines that the citation and referencing style should look like the following: Citation in the text...

22 September 2020 6,897 6 View

What can I do if I get unexpected plasmid after sequencing of bacterial DNA? how can I characterise the unknown plasmid?

After sequencing a bacteria Streptococcus iniae I have got a chromosomal DNA and a extra plasmid DNA. I have not found any evidence of Streptococcus iniae having plasmid!! is it possible to...

02 July 2020 6,319 8 View

How genetic algorithm can be used to find optimal weights and biases of neural network?

Assume a 3-2-1 (3 neurons in the input layer, 2 neurons in the hidden layer and 1 neuron in the output layer) neural network. How genetic algorithm can be used to find optimal weights and biases...

24 May 2020 1,778 2 View

How to extract info from a merged VCF file of annotated WGS analyses in galaxy?

I have a joint flagged tab VCF in which are reported the annotations and variants of 69 samples. How can i extract information for a single sample or chromosome in Galaxy?

21 April 2020 3,836 1 View

What would happen in the chromosome if you had a single crossover between homologous parts of linearised plasmid into chromosome?

The plasmid being used is pBS1 and it has 2 segments of DNA on it which are derived from the B.subtilis amylase gene. There is a chloramphenicol resistance marker inserted between the amyfront and...

18 March 2020 8,875 2 View