I'm trying to replace pili gene of Synechococcus elongatus PCC 7942 with pilA gene of Geobacter sulfurreducens PCA.

I know that S. elongatus PCC 7942 is naturally competent and chromosome can be edited easily by homologous recombination.

But I'm not sure how to design plasmid.

Could you provide me some advice?

Thank you.

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