Byoungchan Park

4 Questions 1 Answers 0 Followers

Questions related from Byoungchan Park

Is it okay to replace CDS only when I want to change antibiotics resistance gene in plasmid?

Hello all. I want to replace spectinomycin resistance gene to kanamycin resistance gene in pAM2991 plasmid. There is information about CDS of each antibiotics resistance gene in Addgene, but I...

07 September 2021 1,848 0 View

How do you replace an antibiotics resistance gene in plasmid?

Hello all. I have plasmid which contains spectinomycin resistance gene. Is it possible to replace it with kanamycin resistance gene or any other antibiotics resistance gene? Thank you for...

18 August 2021 3,375 4 View

How can you replace a gene of Synechococcus elongatus PCC 7942?

I'm trying to replace pili gene of Synechococcus elongatus PCC 7942 with pilA gene of Geobacter sulfurreducens PCA. I know that S. elongatus PCC 7942 is naturally competent and chromosome can be...

14 October 2020 8,978 1 View

Can bovine blood be stained with heme staining method?

I'm trying to perform heme staining after native page of cytochrome c protein. I know that multi heme protein can be stained by heme staining method such as cytochrome c. Then can bovine blood be...

08 October 2020 8,802 2 View