How can you inactivate or remove antibodies in dengue-infected plasma without inactivating the virus?

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Increasing Area Ratio on Quality control Standard on LCMS ?

Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...

08 July 2024 3,150 2 View

What is the Best Midiprep Plasmid Isolation Kit?

Hello, I want to order Midiprep plasmid isolation kit, but I can't decide which brand to use. Can you recommend the brands you always use and think are highly effective? How about GeneJet? Thank You.

30 June 2024 5,865 2 View

Where can we get few mm diameter polished circular glass plates?

who retails few mm diameter polished circular glass plates ? thickness 0.1 to 1 mm diameter 1 to 3 mm ordinary or fused silica glass polished on both sides and the edge

16 June 2024 763 1 View

How to get year-wise Number of Patent filings by Indian listed companies??

I am looking for a way to get the number of patents granted and the number of patents filed by each listed Indian company for the past ten years. Is there any website or database that provides the...

31 May 2024 7,201 1 View

How many percent of specific antibodies in serum after rabbit immunization ?

Hi all, I'm trying to validate an ELISA against anti-drug antibodies in which I use a immunized rabbit serum as positive control. Rabbit was a NZR, immunization protocol was "standard" but I...

13 May 2024 2,828 0 View

What are the extraction methods that can be used to extract formaldehyde component of a fruit e g banana/orange?

AAZ

11 May 2024 7,466 2 View

Is there a link between parental poverty and violence against children ?

Poverty is generally a source of material difficulties. It causes communication difficulties between partners and children. Can it be considered the main source of violence by parents against...

10 May 2024 5,143 3 View

What is the spatial resolution of paleomagnetic data for paleogeographic reconstructions?

Are there any references stating clearly the limitations (especially lower limit) of the spatial resolution of paleomagnetic data when used for paleogeographic reconstructions? i.e., how many...

16 April 2024 8,051 0 View

Hello, any lentivirus vector to permanently transduction of cells for fluorescent cellular membrane?

I am planning to study extracellular vesicles, and to do this I need cells with a fluorescent membrane (GFP, Tomato, etc.), do you guys have any suggestion of lentivirus vector? Thanks!

02 April 2024 1,944 0 View

Identification of aquatic(?) insect found in fountain with dead leaves?

Found this larva in a fountain filled with deciduous leaves in the Jura Moutains of Western Switzerland. Since there were also Trichoptera larvae (Stenophylax, or Micropterna, sequax) feeding on...

30 March 2024 5,350 4 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How to select relevant metabolites from multiple annotations ?

I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...

24 July 2024 5,789 0 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View