Any suggestion in a reliable interneuron molecular marker?

Reading papers I've found out that most interneurons express Calbindin, Calretinin and Parvalbumin, however I've also read that some pyramidal neurons do so as well, so I'm not too sure about...

03 April 2017 3,174 6 View

Company-made custom FISH probes validation?

31 December 2016 5,595 0 View

How to fixate with 95% Ethanol in fresh tissue cryostat sections?

Hi everybody When I get fresh frozen tissue (without fixating with PFA or formaldehyde, just snap frozen with OCT) sliced with the cryostat mounted in a slide it doesn't immediately get attached...

02 March 2016 3,444 1 View

In an In Situ Hybridization, how can someone tell that a probe is labeling a certain gene and ONLY that particular gene?

I know the probes are specific, but as in PCR, sometimes you might be annealing with something else, specially if you designed the probe yourself and its specificity hasn't been validated. How do...

31 December 2015 8,747 3 View

Can someone advise me of an unbiased protocol for sampling the different layers of the brain cortex throughout a different fetal stages?

The thing is, I need to sample the different layers of a sheep cortex at different fetal stages, but I need to do it in an unbiased way, for I have to correlate gene expression from one point of...

31 December 2015 1,670 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View