Sebastian Alonso Quezada

6 Questions 9 Answers 0 Followers

Questions related from Sebastian Alonso Quezada

Any suggestion in a reliable interneuron molecular marker?

Reading papers I've found out that most interneurons express Calbindin, Calretinin and Parvalbumin, however I've also read that some pyramidal neurons do so as well, so I'm not too sure about...

04 April 2017 3,217 6 View

How can you get an rough mental estimate to correlate gene expression and FISH fluorescence?

I want to conduct FISH for some genes of my interest, and I need to determine whether I'll need probes for normal or extra sensitivity (which are more expensive than the normal ones). I've started...

03 March 2017 6,922 1 View

Company-made custom FISH probes validation?

Hi everyone =) I already know that if you make your FISH probes yourself you have to undertake the painstaking process of validating them so they don't cross-react with other things and stuff and...

01 January 2017 5,632 0 View

How to fixate with 95% Ethanol in fresh tissue cryostat sections?

Hi everybody When I get fresh frozen tissue (without fixating with PFA or formaldehyde, just snap frozen with OCT) sliced with the cryostat mounted in a slide it doesn't immediately get attached...

03 March 2016 3,478 1 View

Can someone advise me of an unbiased protocol for sampling the different layers of the brain cortex throughout a different fetal stages?

The thing is, I need to sample the different layers of a sheep cortex at different fetal stages, but I need to do it in an unbiased way, for I have to correlate gene expression from one point of...

01 January 2016 1,706 3 View

In an In Situ Hybridization, how can someone tell that a probe is labeling a certain gene and ONLY that particular gene?

I know the probes are specific, but as in PCR, sometimes you might be annealing with something else, specially if you designed the probe yourself and its specificity hasn't been validated. How do...

01 January 2016 8,786 3 View