How can you explain the appearance of this second half sigmoid?

17 October 2023 1 10K Report

One sigmoid is clearly visible on the ITC titration curve, but half of the second sigmoid is also visible at the beginning of the curve.

Adam B Shapiro

Make sure you deleted the first addition from the analysis, as this is usually not of sufficient quality. Then, see whether it is a reproducible phenomenon. If so, then consider the possibility that either the ligand or the protein is a mixture or, in the case of the protein, not homogeneous. For example, run the protein through a gel filtration column to remove aggregated protein.

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