Hello Vikas, hope all's well.

This is an interesting question but I think we need more information please. How do you wish to measure (mass spectrometry? fluorescence? UV-Vis spectrometry? etc) the products of protein digest i.e. peptides or amino acids?

There are numerous discussions on this site dealing with the theoretical "total" amount of protein (whether in moles or gram amount) in a single cell. From what I know, the hurdle isn't digesting that amount of protein as you could serially dilute an enzyme (for example trypsin or whatever your enzyme of choice would be) solution and then add it to the well/tube containing the single cell protein amount.

The hurdle is ultimately measuring the protein digest products in the background of DNA, RNA and other cellular debris.

Any further information gratefully received.

Kind regards, Peter

Hello Sir,

Thank you very much for your valuable suggestion. I am still planning to carry research on this field. UV-Vis spectrometry will be used for the study to measure the product of protein digested that is amino acids. Actually I am planning to incorporate the single cell protein the foods and then want to check their digestibility. Due to the lack of the proper infrastructure it would not be possible for me to measure the protein digest products in the background of DNA, RNA and other cellular debris.

Thanking you once again for the kind suggestion. Any further suggestion in this regard is highly appreciable.

Use pH Stat.

... or cell digestión device, using your cells as sustrate and measuring protein digestion products by OPA.

HNS