I’m working on screening for locally found ureolytic bacteria strains isolated from soils.
I am having challenges understanding a certain part of the protocol which is being used to test urease activity with the aid of the conductivity meter. I would like you to assist me with this problem.
If conductivity reading (mS) was taken every minute for a duration of 5 min and was used to generate an average change in conductivity (mS/min) and is used to determined urea activity which is mM urea hydrolysed.min-1 = Conductivity(mS/min) x 11.11 at 25˚C And specific urease activity was calculated by dividing urea activity by optical density @ 600nM , what OD value should be used in the calculation to determine specific urease activity?.. Should OD be taken after conductivity reading is done at 5mins or a OD should be measured every min and an average OD value was be used?
Dear Prof. Tarik,
Thanks very much for your response. it was very informative and helpful.
Yes, ureolytic isolates are gaining strong interest in biocementation especially by civil engineers as an alternative means to cementation. in my research i aim to screen and isolate bacteria from soils which high potentials for urease enzyme production like S. pasteurii
Dr. Salwa Al-Thawadi of University of Bahrain also provided an insight on this question i posed. He suggested i check the OD reading of the culture after completion of incubation period and measurement of conductivity with this readings i can then calculate specific urease activity.
If i may ask, in the experiment of biogrout test, based on your suggestion of using "bang media' to culture S. pasteurii, do you think its suitable to increase pH of the media to 8.5 for culturing of bacteria ?
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