Hi Shalley!

What are you trying to achieve ?

CRISPR Cas systems can do:

1. DNA gene Knockout

2. DNA bases Knockin

3. Activation of expression of a gene

4. Inhibition of expression of a gene

5. RNA cleavage

6. Base editing

7. Methylation

8. Tagging

9. Genome wide/ targeted screens

etc..

Are you looking to insert a gene using this ?

The question wasn’t very clear.. Please elaborate.

Yes I want to insert a transporter gene from a pentose fermenting yeast strain to another yeast model for its efficient expression.

Hi there,

CRISPR/Cas9 can certainly be used for that purpose. Make sure that your gene of interest is as the end under the control of a yeast promoter and that insertion site presents no risk for cell viability after insertion.

Hi Dominique,

Thanks! Could you cite any research paper describing the similar problem?

Hi there,

I have started with CRISPR/Cas9 in S. cerevisiae using the excellent system described in the following paper. The main advantage is that all the strains and plasmids used in the paper are available from Euroscarf and protocols are detailed. Article CRISPR/Cas9: A molecular Swiss army knife for simultaneous i...

Thank you Dominique!

Please check my paper on transport engineering

Article Transporter Engineering for the development of Cyanobacteria...