Shalley Sharma

11 Questions 15 Answers 0 Followers

Questions related from Shalley Sharma

Protocol to measure yeast growth curves and eGFP fluorescence simultaneously?

Hi everyone, I’m looking for a reliable protocol to measure yeast growth curves and eGFP fluorescence simultaneously using a ThermoScientific Varioskan LUX plate reader. If anyone has experience...

20 June 2025 1,808 4 View

Can I use Saccharomyces CYC1 terminator for Pichia?

Is it possible for me to use CYC1 terminator from Saccharomyces cerevisiae for use in Pichia pastoris system ? What all parameters are needed to be considered?

19 October 2022 408 0 View

Are there any commercial strains of Saccharomyces for the recombinant protein production ?

I would like to know the culture collections or other private institutions who could provide yeast strains for protein production possessing strong promoters, selection markers and possessing...

17 February 2022 9,758 6 View

How can we use crispr cas technology for transporter engineering in yeast?

I wish to know if this latest technology could be employed for heterologous expression of transporter genes in yeast.

25 May 2020 2,565 7 View

Hi! I would like to know what should be the Ct value (RT-PCR)of 18S housekeeping genes?

I have used 18S primers as housekeeping genes for my yeast strains. These are showing 1.5-2.0 CT value on RT-PCR while genes under study are showing CT value in higher range from 23-30. I also...

27 April 2018 8,073 9 View

Has anyone fused yeast protoplasts through electroporation using PEG?

I need to know the program (voltage, time, capacitance and resistance) for this process.

24 March 2017 2,722 1 View

How can we reduce fluctuations in the readings of Xylose Reductase enzyme activity assay performed at 340 nm?

While performing the enzyme activity of XR and XDH at 340 nm, during time scan of 3 mins, my readings fluctuate invariably. Is there any certain factor to be taken care of while putting up the...

09 August 2016 5,384 3 View

What is Codon Optimization?

There are web tools to optimize your gene of interest but they do not apply to my organism of interest (e.g. in Jcat). How can I optimize my gene's codons ?

10 March 2016 6,359 3 View

Is there any quick and inexpensive way of estimating residual glucose and xylose concentration during fermentation apart from DNS ?

I am performing co-fermentation experiment with glucose and xylose and to determine the residual sugar concentration I generally prefer HPLC method but HPLC is not in work for a few days. Can...

26 June 2015 3,038 6 View

What Is the exact protocol for estimation of reducing sugars using DNS ?

I have been using 10g NaoH, 10 g DNS, 2g Phenol and 0.2 g Rochelle salt. Later Sodium sulfite is being added to the stock solution while preparing the working solution. But dark brown to black...

03 June 2015 4,587 9 View

What is the percentage of hexoses and pentoses in lignocellulosic biomass?

Upon hydrolysis of the lignocellulosic biomass, what percentage of hexose and pentose sugars are obtained?

14 November 2014 2,046 3 View