How can we use 3` and 5` RACE in cloning of potyvirus?

Lalit Meena @Lalit_Meena

12 December 2014 1 8K Report

The terminal portion of the RNA can be identified but how can I design primer of middle part of the RNA?

Juan David Ospina Villa

well, first one you need to know the sequence. 3'RACE only can be used in organisms or genomes previously sequenced.

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Science method

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