Lalit Meena

5 Questions 13 Answers 0 Followers

Questions related from Lalit Meena

How can we use 3` and 5` RACE in cloning of potyvirus?

The terminal portion of the RNA can be identified but how can I design primer of middle part of the RNA?

12 December 2014 8,441 1 View

Hi, I want to clone the full length of potyvirus which is a positive single stranded RNA with a size of 9.7 kb. How can I clone it?

Since the size is a bit larger (9.7 kb) I have to clone it in fragments (subcloning). Overlap primers can be designed for PCR amplification.

12 December 2014 7,851 3 View

How can I clone 9.7kb single stranded positive rna of potyvirus ?

i have designed degenerate primer and amplified product (700 bp) is cloned and sequenced. but now how can i proceed for for cloning of rest (9.0kb) of the the genome.....

12 December 2014 7,138 5 View

What is the exact reason behind the high polydispersity index of natural polymeric nanoparticles in comparison to synthetic polymeric nanoparticles?

Is it true in all cases of NPs formulations or not?

03 March 2014 1,196 2 View

How can we separate and identify unknown or not reported microRNA from isolated microRNA mixture? How qPCR will be helpful?

micro RNA identification

09 September 2012 423 8 View