How can we test the presence of Bacteria?

More Arun P Jose's questions See All

How to remove the air bubbles in comercially available ultrasound gel?

The ultrasound gel which is commercially available contains lot of air bubbles.Which creates lot of noise while scanning. How to avoid the air bubbles in the gel such that the scan wont have much...

11 December 2019 9,946 2 View

How to specify material properties for Eulerian-Eulerian multiphase model in fluent ?

I am analyzing the copper water nano fluids in fluent by multi phase Eulerian-Eulerian approach.i want to describe the material properties of copper nano paricle which are dispersed in water.i...

03 April 2016 6,204 14 View

What will be vibrational frequency exerted by the road on the tyres?

I am designing a wheel chair i want to test my design using ansys workbench harmonic response.So what are the parameters i should describe, especially the frequency range for the surface...

03 April 2016 8,893 2 View

What is the viscosity of copper nano particles?

I am doing a simulation in fluent i am using eulerian -langragian model i want to provide the material properties for secondary phase which is copper nano particle

03 April 2016 2,753 2 View

How to specify volume concentration in ansys fluent 16???

I am currently doing a project on convective heat transfer in nanofluids.my geometry is a 2D circulartube. how to specify the volume concentration of base fluid and secondary fluid in multiphase...

02 March 2016 3,060 4 View

How to plot the fluid temperature and wall temperature of a fluid flowing through a pipe in ansys fluent 16??other than taking locations

laminar flow of water is considered

02 March 2016 8,435 0 View

High speed flows can be simulated with what type of solver that disregards viscosity?

01 February 2016 7,004 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

How do you decide an optimal AC amplitude for non-faradaic electrochemical impedance spectroscopy?

I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...

16 July 2024 5,031 1 View

Can I use candle-jar technique to lactic acid bacteria?

I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria

15 July 2024 8,890 2 View

What is the behavior of anaerobic bacteria in deep salty groundwater ?

What is the behavior of anaerobic bacteria in deep salty groundwater with high level of chlorine? Is the nitrate level deceasing and is denitrification controlled by excess chlorine? Thanks

13 July 2024 1,581 4 View

Alireza Mordadi

Use different bacteria with different concentration for contamination product,then do your test with UV and then do colony count.

Yehia A.-G. Mahmoud

put the fabricated product in environment ithat lead to contamination, like high humidity that might encourage fungi to grow on it, suitable temp. about 25-37oC, and monitor your results

Alaa Kareem Niamah

Hi, you can use ATP determination in growth media after UV treatment.