How to start a small vermicompost or organic farming business and where to get funds in India?

In this time where organic farming is the best and only option for future sustainability in terms of griculture there is a need to go for biofertilizers and vermicompost techqniques and to use...

22 May 2020 5,934 14 View

What is the best mobile phase for separation of caffeine by HPTLC system?

I have used dichloromethane : methanol = 90 : 10 but the caffeine got eluted with the mobile phase. Both the chemicals used were of HPLC grade (Merck).

17 June 2019 6,627 7 View

What concentration range should I use to build standard curve for ortho-nitro phenol ?

I would also like to know the linear range of this assay and the detection range? This will be used to measure end point production from beta-gal assay.

26 March 2018 1,546 3 View

How to find Throughput of an Optical Interconnection Link ?

06 March 2017 1,291 4 View

Any scientist working in the field of Biofertilizer on crops and what are the effects of bioconsortium on rice and maize throughout the world

I am a Research scholar on Biofertilizer .

19 May 2016 5,981 1 View

Does anyone have a working protocol for FISH for telomere probe of the cells growing on silicone membrane?

I successfully did FISH for cells growing on cover slip, but the same protocol is not working for cells growing on silicone membrane. Do I need to change the hybridization time, temp or...

15 April 2014 8,324 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

03 March 2021 5,360 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View