Enantiomers
You have to select a chiral column. I think you have to optimised with non-polar hexane, heptane,chloroform.
Which type of compounds you want to separate?
Check this
sorry why do you ask this way? what is your compounds, what is your haplc system what is your knowledge background?
I operate on two systems
1) reversed phase I use macherey nagel columns they have a searchable data base.
Start : http://www.mn-net.com/HPLCStart/SpecialHPLCphases/HPLCenantiomer/tabid/6147/language/en-US/Default.aspx
2) "normal phase" I usually do with regis columns whelk O 1 and 2 are Ok starting points if you do not know anything.
If you have to find out by try and error it will get expensive as these columns cost a bit.
have fun
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