Gyanendra Prasad Panda

4 Questions 6 Answers 0 Followers

Questions related from Gyanendra Prasad Panda

How to dissolve Chitosan methacrylate in milli-Q?

I have prepared Chitosan methacrylate. After its preparation when I tried to dissolve and mix PEGDMA to UV crosslink using IRGACURE, no crosslinking is observed. Can anyone find me a reason for it?

29 May 2025 3,377 1 View

How can we seed cells insitu on algiante-tyramine hydrogels crosslinked via HRP and H2O2?

I need a brief protocol regarding seeding of cells in situ with alginate-tyramine crosslinked hydrogel and taking that cells for Q RT-PCR study after 72 hours. please mention if there is any...

14 November 2022 5,099 2 View

How to perform RNA isolation and RT-PCR for Cells seeded in 3d hydrogels ?

I want to do RNA isolation and RT-PCR of cells seeded on 3d hydrogels with high purity and noninterference of hydrogels. Can you please suggest a detailed protocol to achieve the same?

28 October 2022 2,097 3 View

Purification of fluorescent labelled bisphosphonate ?

Can anyone share the protocol for purification of any fluorescent labelled bisphosphonate drug other than RP-HPLC?

26 February 2022 5,888 6 View