We are trying to run fiber photometry experiments for long time scales, but we get these sinusoidal artifacts that come up. We think they may be caused by twisting of the fiber optic subject cable. We tried using a rotatory joint to alleviate this, but the attenuation it causes is way too high (it attenuates the light coming back from the animal). Another rotatory joint we tried causes too much motion artifact and defeats the purpose of using it. Does anyone know a solution to minimize this type of artifact, or know of a good rotatory joint for photometry?
Dear Jay Phansalkar ,
you might explain a bit more in detail the set up and the animal(s) you are dealing with.
'rotation' and/or 'twisting' of fibre at that high frequency of about 2 Hz during photometry?
What are you doing there with the fiber?
Such cycles in the diagram curve remind me on results when I dealt with fiber optic heartbeat and respiration motion *) detection.
What about respriation motion which causes 'periodic' coupling**) losses?
Mice, rabbits or dogs as animals with quite high frequency respiration cycles?
*) Please have a look at the attachment showing similar structures as yours; taken from: Conference Paper Optical Detection Of Respiration Motion And Heart Beats For ...
**) Slight changes in the distance between the animal and the fiber will give changes in the amount of light picked up by the fiber.
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