How can we know what vaccum pressure is suitable to evaporate methanol chloroform mixture during liposome synthesis?

16 February 2022 0 10K Report

Normally we have guidelines what vaccum pressure is suitable for an individual solvent at particular boiling point when using Rotavapor. I am using chloroform:methanol mixture(2:1) to dissolve lipids, temperature 20°C(40°C water bath). Chloroform and methanol form azeotropes with atm boiling point around 54°C. So, what is the vaccum pressure I can increase upto to form homogeneous lipid film without solvents shooting right away?

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