My Dear 

we used 115-120g for 20 minutes, then we have basically two layers - PRP on top and larger cells on bottom, and you should take the top layer leaving a good centimeter above the erythrocyte layer. Then you are sure to have cleaner PRP preparation and less chance of picking a white blood cell too.

You can read :

Platelet separation and quality control method.docx

Dear Rafid,

You should collect the blood in citrate (one part of citrate and 9 parts of blood. Mix well. Centrifuge the blood at 800 rpm for 20 minutes.  Collect the supernatant platelet rich plasma in a separate tube.  Omer

For PRP preparation Collect 9 ml of venous blood (well taken with minimum of stasis) into a plastic tube containing 1 ml of aqueous tri-sodium citrate anticoagulant, 32 g/l  Mix the blood well with the anticoagulant. Without delay,

centrifuge the blood at 600 g–1000 g( 2000 rpm) for 10 minutes.   Immediately remove the plasma into a plastic tube (vial) and stopper.

for further reading refer to 

Dacie and Lewis Practical Hematology, 

  Author: Lewis.

 Collect fresly venous blood  into 130mM aqueous trisodium  citrate antcoagulant solution(9:1) and mix very well.Centrifuge whole blood at 100xg for 25 minutes.Then collect platelet rich plasma (PRP) into a plastic tube.

The most direct way to consistently prepare PRP as well as PPP and PFP is to use a centrifuge specifically designed for that purpose: The PDQ Platelet Function Centrifuge.  Samples  are prepared exactly the same way time after time.  go to www.biodatacorp.com for details

Hi

This link will help you

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338460/

Regards

I agreed with DR. Omer Iqbal

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