In codon optimization and GC content analysis , softwares show an average GC% of sequence and GC% for each codon too. what is interpretation of GC% for each codon?
Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...
18 July 2024 1,579 2 View
I am doing a RT-qPCR for gene expression analysis of cancer patients, and there are four different groups in the results. The first group that shows over expression of my target gene (both two...
31 May 2024 1,088 0 View
Can anyone interpret why the absorbance (DNS assay method) in alpha amylase assay get increased with increased standard (inhibitor) concentration? it should be decreased with increased standard or...
26 May 2024 4,753 2 View
I attempted a phosphate (apatite) flotation, but the slurry was too thick for the impeller to work properly. I reduced the solid content to 30% for better mixing, but the conditioning still seems...
14 May 2024 4,505 4 View
Hello everyone, recently I am trying to synthesize a dipeptide with a Coumarin derivative of Tryptophan, and the problem is it doesn't contribute in reaction at all. Tryptophan itself in the same...
15 April 2024 6,778 3 View
Hello All, I'm doing RNA extraction from cells. I tried both ways by using TRIZOL (with chloroform, and isopropanol) and RNeasy mini kit. When I use TRIZOL, the RNA concentration is high, however...
11 March 2024 9,171 8 View
Hello All I prepared my library to run for sequencing. I would like to check the quality of my samples through D1000 ScreenTape Assay before add to sequencer. Can anyone help me to interpret my...
10 March 2024 8,764 0 View
Hello all I'm working with SHSY5Y cells to differentiate them to neuronal cells. I'm following the protocol with using RA, BDNF and cAMP for cell differentiation. I tried it different times but...
19 February 2024 1,672 5 View
Hello all I'm working with SHSY5Y cells and I always made Cryo cells from them, them thawed them and used them without problem. But, recently, after thawing, they are remain floating and not...
19 February 2024 9,305 2 View
Hello All, I have shRNA lentivirus particles and I want to produce self-inactivating replication incompetent viral particles in cell by myself. My purpose is to produce knock-down cells in flask...
10 January 2024 6,425 0 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
i am unable to interpret why its increases in start as shown in figure
11 August 2024 2,179 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View