Hi, I am using a miRCURY RNA Isolation Kit to extract exosomal RNA from 0. 5 ml serum and I got very low volume. Then I reduced the elution volume ( from 100 µL to 50 µL) in order to make it more concentrated and I increased the incubation time on the column and heated the water (37C) but still get very small RNA volume. Any advice will be appreciated.

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