Taral R Lunavat

5 Questions 7 Answers 0 Followers

Questions related from Taral R Lunavat

How can I grow U87 and U251 cells in neurospheres in exosome depleted FBS?

We are trying to grow the U251 and U87 cells in Neurosphers. At first when I cultured the cells in DMEM high glucose with 1% PEST, 1% L-Glut and 1% NEAA with 10% regular FBS (non exosome...

25 October 2017 604 0 View

How can I check for phosphorylation of proteins in cell lysates?

Can anyone tell me what would be a wise way to perform an experiment to check for the phosphorylation of protein in the cell lysates? I am treating my cells with melanoma exosomes and would like...

30 March 2015 8,628 7 View

Does the phosphorylation of MAPK proteins alter over time during drug treatment in cancer models?

Does the phosphorylation of MAPK proteins alter over time during drug treatment in cancer models? For instance, if I treat melanoma cells with current line of therapy, then do the pERK and pMEK...

12 March 2015 9,755 5 View

What is the difference between blood exosomes and LDL, HDL?

My concern lies, when I try to isolate and purify with optiprep the exosomal fraction from the blood of melanoma patients, I can see different pellets in the ultracentrifugation. I would like to...

16 October 2014 4,934 3 View

What is the main difference between stem like cancer cells and cancer like stem cells? Are they the same or they are totally different?

I am working on melanoma exosomes and my interest lies to check if there is any initiation of cancer stem like properties.

10 October 2014 4,499 3 View