Is there any biochemical test which can be considered as a confirmatory test for Azotobacter? Please provide your valuable suggestions.
Azotobacter sp. can be identified after plating on specific media (Jansen's). Apart from gram, cyst and flagellar staining; biochemical tests viz. Ammonia Production, IAA production, Phosphate solubilization and polysaccaride production could be performed for the confirmatory test.
Identification of Azotobacter on a Petri Dish from an assorted collection of soil bacetria can be quite daunting since a number of bacteria grow on N free media and produce polysaccarides as Azotobacter does. Most often you may end up confusing Stenotrophomonas as Azotobacter if you rely on your instincts alone. Other closely related bacteria like Azomonas, also have similar growth patterns. A chroococcum ca be distinguished based on its pigment production ( dark brown to black). So rely on the polyphasic approach to confirm Azotobacter from soil samples.
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