How can we freeze dry using liquid nitrogen?

More K.P. Annamalai's questions See All

Every star like the sun is at a stationary position. Am I right?

The earth has a orbit, but not for the sun. Similarly, all planets have orbits, but not for stars like the sun.

18 July 2024 1,768 4 View

Whether the gravitational force between two objects (e.g: planets) attracts towards its centers of planets or acts as curvature?

My answer: the gravitational force between two bodies/planets attracts towards its centers of planets.

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Is the sunlight visible in space ?

The sunlight is not visible in the space because there is no particles in space to scatter and reflect the sunlight. Am I right?

14 May 2024 7,444 3 View

What is the difference between gravitation and gravity?

Gravitation is the attractive force between any two planets. Gravity is the force that pulls objects (within the Earth and its Atmosphere) toward the center of the Earth. Am I right?

23 April 2024 4,076 1 View

The whole water in the Earth's oceans are stable. Is this due to the gravity?

How does the gravity work in the Earth?

29 July 2023 6,271 4 View

Does the gravity of any massive object act towards the center of the object or as curvature ...?

Does the gravity of any revolving planet act towards the center of the planet or act as curvature towards the orbit of the planet revolving around its star?

23 July 2023 4,226 13 View

Einstein's mass-energy equivalence is just one value for the velocity in kinetic energy equation. Am i right?

E = (mv^2)/2 is the kinetic energy equation. Here, sqrt(2) * c = 423970560 m/s, where c = 299792458 m/s is speed of the light in vacuum. E = (m*[sqrt(2) * c]^2)/2 = mc^2. ER=m*(423970560 m/s)^2,...

21 July 2023 3,550 6 View

Anything that has mass also has gravity. Is it true?

Anything that has mass also has gravity. Objects with more mass have more gravity. Gravity also gets weaker with distance. So, the closer objects are to each other, the stronger their...

16 July 2023 6,905 59 View

What is wrong in the relativity theory?

The gravity of earth is always attracting all the things(including objects in motion) within the earth's gravitational field towards the center of earth.

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In the Universe, all the objects (except running in vacuum) are orbiting other object(s)or moving in a circular path. Why?

I think these phenomena follow the scientific facts defined in the classical mechanics, but not theories or definitions in Einstein's mechanics.

01 July 2023 1,254 9 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

Melt curve problem in qPCR?

Do these peaks in the curve indicate primer dimer or non-specific products?

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What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

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How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

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Is the black sediment consider as sand? If not, how can I filter it out?

Hi researchers! I'm working on soil texture analysis, and the end result for sand is doubtful because there is black sediment appearing after drying, as shown in the figure. Is it considered sand?...

30 July 2024 557 2 View

Where to start the Analytical model for Dry sliding wear of metal surface ?

I am doing research on wear mechanism of the additive manufactured metal parts under dry condition. Please share the reference to start the analytical modelling of the wear prediction.

29 July 2024 6,115 0 View

Issues with Malaria Coated Nitrocellulose Membrane Releasing Moisture?

Hello, I am currently working with Malaria Pf/Pan nitrocellulose membranes coated for malaria detection. After drying the coated membranes in an Incubator (overnight at 37°C), I've noticed that...

16 July 2024 7,937 1 View

Why does total carboydrate content decrease when increasing the vacuum degree?

I have conducted a survey on the "effect of vacuum drying conditions on carbohydrate content of angelica.S". The result show that, when increasing the vacuum degree, the carbohydrate content first...

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How to Freeze Embryos for Spatial Transcriptomics?

Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...

10 July 2024 5,985 0 View

How to convert ppm to μmoles/g?

Hello, I am trying to convert to convert ppm of a solution to μmoles/g of dry sample.

09 July 2024 7,081 1 View

Mugyeom Choi

I also wonder how to do it. if you have found the method and don't mind, can you send the method to my email? my email is [email protected]

thank you!

Marilyn Mary Xavier

If you found the method could you please share with me??? I am in most need of it...

Archana Sahu

we also would like to know the freeze drying method using liquid nitrogen how to go for cyclodextrin complexes in ethanol?

Najeeb Ullah

K.P. Annamalai , could you please share the protocol here. Thanks

Mohsen Heydari

In certain food freeze dryers, the freezing of the product is also accomplished by spraying liquid nitrogen in the drying chamber in which the product is placed.