Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Do Fire damages Concrete Structures Below soil?

Hi All, I am working on the rehabilitation of a fire-damaged structure. Should I consider the Column below Soil structurally deficient. visually it doesn't look like fire affected the column...

12 January 2021 5,401 3 View

I have generated a figure "Gene distribution by conservation" from 20 genomes of bacteria. Can someone explain how to interpret correctly?

I have generated a figure "Gene distribution by conservation" from 20 genomes of bacteria. Can anybody explain how to interpret it correctly? Thank you in advance

11 January 2021 3,346 2 View

How to address the Irrigation and crop management practices database in SWAT model during simulations?

Dear Colleagues I am using the SWAT model in an irrigated basin where I wanna specify the Irrigation database for major crops (sugarcane, maize, rice, wheat, and cotton) in SWAT model...

03 January 2021 3,923 4 View

Can cosine of solar zenith angle be negative?

I am trying to find the cosine of sun elevation angle on slope terrain, I found the values between -0.9 to 0.9. Should the value be set to 0 - 0.9 or the negative value acceptable?

24 December 2020 4,027 4 View

How can we draw the adsorption isotherms of Langmuir and Freundlich together with the experimental data in a single graph on Excel?

Thanks in advance

22 December 2020 7,853 4 View

How to make a model of trachea (human airway)?

I want to carry out flow analysis of air through tachea (human airway). How can I draw it in a Solidworks after taking a CT Scan image?

14 December 2020 5,031 2 View

How to prepare wheat grain sample for amino acid analysis through UV-HPLC ?

how to prepare wheat grain sample for amino acid analysis through UV-HPLC, and also amino acid standards for HPLC , I will be very very thank full.

10 December 2020 1,207 3 View

Is there a way to implement Kalman filter on three dimensional GPS data to remove noisy data?

I am trying to implement Kalman filter to get rid of multipath error points on GPS data while my data contains Latitude, Longitude, accuracy, altitude, speed and time. I am using python but unable...

01 November 2020 8,837 3 View

How to calculate thermal stress in cylinder if water is flowing inside the cylinder?

How to calculate thermal stress in stainless steel cylinder if water is flowing inside the cylinder?

14 October 2020 905 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Is the a way to contol flow (Flow velocity) without impacting pressure value?

I have a pipe with 10 mm in Diameter and 2 bar pressure, the fluid velocity is 200 m/s, the fluid is air. is there a way to reduce velocity without affecting the pressure value?

25 February 2021 7,187 3 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate time-averaged fields from Ansys CFX solver?

I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...

21 February 2021 8,548 7 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View