1- Absorption of 1 cc of a nm solution of 20 gold particles at 530 nm equals one. Calculate the extinction coefficient of these nanoparticles (consider the light path length of 1 cm).
If we were to put 10 DNA molecules on each of these gold nanoparticles, what would be the required concentration of DNA molecules?
If the reaction volume is 1 mL, how many gold nanoparticles and how many nucleic acid particles will enter the reaction and finally how many “gold nanoparticles associated with DNA”?
2- Dissolve 20 mg of 40 kDa protein in a 100 mm cc phosphate solution. Calculate the concentration of the resulting solution based on molarity and parts per million.
If this protein becomes dimeric, does its concentration change based on molarity?
3-How to prepare a liter of solution containing 0.2 sulfuric acid nitrogen, 0.01 mmol of substance A, 5% v / v of substance B and 20% f / h of substance C?
MW A = 50
MW b = 712
MW C = 240
The molarity of concentrated sulfuric acid is 18.4.
4- There is a substance (4 g / liter) in the solution. The cuvette is 2 cm long and only 50% of the given beam of light transmits. What is the extinction coefficient? What is the molar absorption coefficient if the molecular weight is 100?
5- The absorption coefficient of glycogen and iodine is 0.20 at 450nm light. What is the focus when the transmission is 40% in a 2 cm cuvette?
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