I want to calculate osmolarity of 10%PEG in media (yeast extract 0,6%, Mannitol 2%). I want to make a hypotonic buffer to get compatible solutes from bacterial pellet by milking. I need mosm/l of media first to make hypotonic solution. Can anyone help me?
PEG6000 does not ionizes in solution, i.e. is not divided into their atomic constituents but it is still a single molecule. Therefore, its molarity is equal to its osmolarity, i.e. If we say that it is 3 M, its osmolarity is 3 OSM (osm/l). In the case of 10% PEG in media, it means that in 1000 l of media we have 100 ml of PEG. So, if average MW of PEG 6000 is 6000 g/mol we can say that your solution is 0.016 M, or 0.016 osm/l=16 mosm/l
dear Jose Maria Saugar
you cleared in a very gentle way but still i could not understand how the molarity or the osmolarity of 0.016 comes
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